Drk-mediated signaling to Rho kinase is required for anesthesia-resistant memory in .
Τίτλος | Drk-mediated signaling to Rho kinase is required for anesthesia-resistant memory in . |
Publication Type | Journal Article |
Year of Publication | 2017 |
Authors | Kotoula, V., Moressis A., Semelidou O., & Skoulakis E. M. C. |
Journal | Proc Natl Acad Sci U S A |
Volume | 114 |
Issue | 41 |
Pagination | 10984-10989 |
Date Published | 2017 10 10 |
ISSN | 1091-6490 |
Λέξεις κλειδιά | Actins, Anesthetics, Animals, Animals, Genetically Modified, Cytoskeleton, Drosophila melanogaster, Drosophila Proteins, Drug Resistance, Memory, Mushroom Bodies, rho-Associated Kinases, Signal Transduction |
Abstract | Anesthesia-resistant memory (ARM) was described decades ago, but the mechanisms that underlie this protein synthesis-independent form of consolidated memory in remain poorly understood. Whether the several signaling molecules, receptors, and synaptic proteins currently implicated in ARM operate in one or more pathways and how they function in the process remain unclear. We present evidence that Drk, the ortholog of the adaptor protein Grb2, is essential for ARM within adult mushroom body neurons. Significantly, Drk signals engage the Rho kinase Drok, implicating dynamic cytoskeletal changes in ARM, and this is supported by reduced F-actin in the mutants and after pharmacological inhibition of Drok. Interestingly, Drk-Drok signaling appears independent of the function of Radish (Rsh), a protein long implicated in ARM, suggesting that the process involves at least two distinct molecular pathways. Based on these results, we propose that signaling pathways involved in structural plasticity likely underlie this form of translation-independent memory. |
DOI | 10.1073/pnas.1704835114 |
Alternate Journal | Proc. Natl. Acad. Sci. U.S.A. |
PubMed ID | 28973902 |
PubMed Central ID | PMC5642687 |