Targeted profiling of hydrophilic constituents of royal jelly by hydrophilic interaction liquid chromatography-tandem mass spectrometry.
| Τίτλος | Targeted profiling of hydrophilic constituents of royal jelly by hydrophilic interaction liquid chromatography-tandem mass spectrometry. |
| Publication Type | Journal Article |
| Year of Publication | 2018 |
| Authors | Pina, A., Begou O., Kanelis D., Gika H., Kalogiannis S., Tananaki C., Theodoridis G., & Zotou A. |
| Journal | J Chromatogr A |
| Volume | 1531 |
| Pagination | 53-63 |
| Date Published | 2018 Jan 05 |
| ISSN | 1873-3778 |
| Λέξεις κλειδιά | Chromatography, High Pressure Liquid, Fatty Acids, Food Analysis, Hydrophobic and Hydrophilic Interactions, Limit of Detection, Tandem Mass Spectrometry |
| Abstract | In the present work a Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry (HILIC-MS/MS) method was developed for the efficient separation and quantification of a large number of small polar bioactive molecules in Royal Jelly. The method was validated and provided satisfactory detection sensitivity for 88 components. Quantification was proven to be precise for 64 components exhibiting good linearity, recoveries R% >90% for the majority of analytes and intra- and inter-day precision from 0.14 to 20% RSD. Analysis of 125 fresh royal jelly samples of Greek origin provided useful information on royal jelly's hydrophilic bioactive components revealing lysine, ribose, proline, melezitose and glutamic acid to be in high abundance. In addition the occurrence of 18 hydrophilic nutrients which have not been reported previously as royal jelly constituents is shown. |
| DOI | 10.1016/j.chroma.2017.11.019 |
| Alternate Journal | J Chromatogr A |
| PubMed ID | 29198446 |
