Aberrant p16 promoter methylation among Greek lung cancer patients and smokers: correlation with smoking.
Τίτλος | Aberrant p16 promoter methylation among Greek lung cancer patients and smokers: correlation with smoking. |
Publication Type | Journal Article |
Year of Publication | 2007 |
Authors | Georgiou, E., Valeri R., Tzimagiorgis G., Anzel J., Krikelis D., Tsilikas C., Sarikos G., Destouni C., Dimitriadou A., & Kouidou S. |
Journal | Eur J Cancer Prev |
Volume | 16 |
Issue | 5 |
Pagination | 396-402 |
Date Published | 2007 Oct |
ISSN | 0959-8278 |
Λέξεις κλειδιά | Adult, Aged, Bronchi, DNA Methylation, Female, Genes, p16, Humans, Lung Neoplasms, Male, Middle Aged, Polymerase Chain Reaction, Promoter Regions, Genetic, Sex Characteristics, Smoking, Sputum |
Abstract | Genetic and environmental factors (dietary and smoking) influence lung cancer epidemiology and induce epigenetic modifications that should be assessed in individual populations. We analyzed p16 methylation among Greek non-small cell lung carcinoma patients and smokers using two-stage methylation-specific polymerase chain reaction. One hundred and fifty specimens from cancerous and adjacent non-cancerous tissue, bronchial washings and sputum from patients and 48 specimens, mostly sputum, from disease-free smokers were included. p16 methylation was very frequent in biopsies (82.85%) and bronchial washings (non-small cell lung carcinoma, 80.35%; small cell lung carcinoma, 16.66%) from patients, but also in adjacent non-cancerous tissue (45.71%). Concordance of p16 methylation and positivity by cytological examination was 51.78%. Methylation was also observed in sputum from asymptomatic cytology-negative smokers (22.5%) and chronic obstructive pulmonary disease patients (three of eight). Among disease-free individuals, methylation correlated only with heavy smoking (>50 pack-years, P<0.001) and differed among male and female disease-free smokers. In summary, p16 methylation is very frequent among non-small cell lung carcinoma patients, and correlates with heavy cigarette consumption only in disease-free smokers. |
DOI | 10.1097/01.cej.0000236260.26265.d6 |
Alternate Journal | Eur J Cancer Prev |
PubMed ID | 17923809 |