Bone Marrow work-up: report of a pilot study.
Τίτλος | Bone Marrow work-up: report of a pilot study. |
Publication Type | Journal Article |
Year of Publication | 2015 |
Authors | Anagnostopoulos, I., Lenze D., Hummel M., Dietel M., & Jöhrens K. |
Journal | Recent Results Cancer Res |
Volume | 199 |
Pagination | 95-105 |
Date Published | 2015 |
ISSN | 0080-0015 |
Λέξεις κλειδιά | Biopsy, Bone Marrow, Clonal Evolution, DNA, Humans, Immunoglobulin Heavy Chains, Immunohistochemistry, In Situ Hybridization, Fluorescence, Pilot Projects, Polymerase Chain Reaction, Quality Control, Specimen Handling |
Abstract | In this pilot study we changed several pre-analytical variables of bone marrow trephine biopsy processing with the task to achieve not only a preservation of morphology and antigens but also of nucleic acids. The changes involved employment of a newly established decalcification solution in conjunction with a short fixation time (2 h after receiving the specimens) and performance of decalcification at 37 °C. The comparison of the obtained results from three specimens with those of our routinely established protocol unequivocally revealed that the novel decalcification solution results in a superior preservation of nucleic acids, with only slight differences in preservation of morphology and cellular antigens. These encouraging results imply that this novel decalcification solution will allow a widely accepted standardization of bone marrow trephine biopsy processing. |
DOI | 10.1007/978-3-319-13957-9_10 |
Alternate Journal | Recent Results Cancer Res. |
PubMed ID | 25636433 |