HILIC-MS/MS Multi-Targeted Method for Metabolomics Applications.
Τίτλος | HILIC-MS/MS Multi-Targeted Method for Metabolomics Applications. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Virgiliou, C., Gika H. G., & Theodoridis G. A. |
Journal | Methods Mol Biol |
Volume | 1738 |
Pagination | 65-81 |
Date Published | 2018 |
ISSN | 1940-6029 |
Λέξεις κλειδιά | Animals, Biomarkers, Chromatography, High Pressure Liquid, Humans, Hydrophobic and Hydrophilic Interactions, Metabolomics, Tandem Mass Spectrometry |
Abstract | Metabolomics aims at the identification and quantification of key-end point metabolites, basically polar, in order to study changes in biochemical activities in response to pathophysiological stimuli or genetic modifications. Targeted profiling assays have enjoyed a growing popularity during the last years with LC-MS/MS as a powerful tool for development of such (semi-) quantitative methods for a large number of metabolites. Here we describe a method for absolute quantification of ca. 100 metabolites belonging to key metabolite classes such as sugars, amino acids, nucleotides, organic acids, and amines with a hydrophilic interaction liquid chromatography (HILIC) system comprised of ultra (high) performance liquid chromatography (UHPLC) with detection on a triple-quadrupole mass spectrometer operating in both positive and negative electrospray ionization modes. |
DOI | 10.1007/978-1-4939-7643-0_5 |
Alternate Journal | Methods Mol Biol |
PubMed ID | 29654583 |