Induction of apoptosis in CD4(+) T-cells is linked with optimal treatment response in patients with relapsing-remitting multiple sclerosis treated with Glatiramer acetate.
Τίτλος | Induction of apoptosis in CD4(+) T-cells is linked with optimal treatment response in patients with relapsing-remitting multiple sclerosis treated with Glatiramer acetate. |
Publication Type | Journal Article |
Year of Publication | 2019 |
Authors | Boziki, M., Lagoudaki R., Melo P., Kanidou F., Bakirtzis C., Nikolaidis I., Grigoriadou E., Afrantou T., Tatsi T., Matsi S., & Grigoriadis N. |
Journal | J Neurol Sci |
Volume | 401 |
Pagination | 43-50 |
Date Published | 2019 Jun 15 |
ISSN | 1878-5883 |
Λέξεις κλειδιά | Adult, Apoptosis, CD4-Positive T-Lymphocytes, Female, Flow Cytometry, Follow-Up Studies, Glatiramer Acetate, Humans, Immunosuppressive Agents, Interferon-beta, Male, Middle Aged, Multiple Sclerosis, Relapsing-Remitting, Treatment Outcome |
Abstract | BACKGROUND: Induction of T-cell apoptosis constitutes a mechanism of action for Glatiramer Acetate (GA). We investigated whether activation of apoptotic T-cell death may be indicative of optimal treatment response in patients with relapsing-remitting Multiple Sclerosis (RRMS), with respect to radiological activity.METHODS: We studied apoptotic markers on blood T-cells of forty patients with RRMS, 19 patients under GA and 21 patients under interferon-β (IFNβ), by flow cytometry. Patients were relapse-free and were further classified into optimal and sub-optimal responders based on radiological activity. Eighteen patients (8 patients under GA and 10 patients under IFNβ were additionally evaluated at a 12-month follow-up and were relapse- and radiological activity-free. For these patients, apoptosis was also evaluated by molecular techniques.RESULTS: At inclusion, optimal responders to GA exhibited increased (23.6 ± 1.976) relative % frequency of CD4(+)AnnexinV(+)7AAD(-) T-cells, compared to sub-optimal responders (14.478 ± 1.204, p = 0.001). Similarly, relative % frequency of caspase-3(+) T-cells was 1.517 ± 0.436 versus 0.45 ± 0.149 (p = 0.041), respectively. Anti-apoptotic molecule bcl-2 showed an inverse pattern 4.532 ± 1.321 versus 13.094 ± 3.987, p = 0.044, respectively. These differences were not evident for IFNβ-treated patients.CONCLUSIONS: T-cell apoptotic markers may be applied as a biomarker useful in evaluating optimal treatment response under GA, thus allowing for personalized treatment decisions. |
DOI | 10.1016/j.jns.2019.03.030 |
Alternate Journal | J Neurol Sci |
PubMed ID | 31009931 |