Isolation and characterization of a third isoform of human hepatocyte nuclear factor 4.
Τίτλος | Isolation and characterization of a third isoform of human hepatocyte nuclear factor 4. |
Publication Type | Journal Article |
Year of Publication | 1996 |
Authors | Kritis, A. A., Argyrokastritis A., Moschonas N. K., Power S., Katrakili N., Zannis V. I., Cereghini S., & Talianidis I. |
Journal | Gene |
Volume | 173 |
Issue | 2 |
Pagination | 275-80 |
Date Published | 1996 Sep 16 |
ISSN | 0378-1119 |
Λέξεις κλειδιά | Amino Acid Sequence, Animals, Basic Helix-Loop-Helix Leucine Zipper Transcription Factors, Cell Line, Chromosome Mapping, Chromosomes, Human, Pair 20, DNA, DNA-Binding Proteins, Gene Expression, Hepatocyte Nuclear Factor 4, Humans, Liver, Molecular Conformation, Molecular Sequence Data, Phosphoproteins, Protein Binding, Rats, Sequence Homology, Amino Acid, Tissue Distribution, Transcription Factors, Transcriptional Activation |
Abstract | Hepatocyte nuclear factor 4 (HNF-4) is an essential positive regulator of a large number of liver-specific genes. We report here the isolation of three HNF-4 isoforms from a human liver cDNA library. hHNF-4A and hHNF-4B, differing by the insertion of 10 amino acids in the C-terminal region, have been previously identified in mouse, rat and human liver. The novel isoform, hHNF-4C, is identical to hHNF-4A and B in the regions encompassing the DNA-binding and dimerization domains, but contains a different C-terminal domain. Similar to the other isoforms, hHNF-4C is produced in a limited number of tissues and represents 2.6-13% of the total hHNF-4 mRNA population, depending on the cell type. The chromosomal origin of all three isoforms has been localized to human chromosome 20. hHNF-4C can form heterodimers with hHNF-4A and B in vitro, and exhibits similar transactivation potential as hHNF-4A or B in transient transfection assays, suggesting that the divergent C-terminal region is not part of the transactivation domain. |
DOI | 10.1016/0378-1119(96)00183-7 |
Alternate Journal | Gene |
PubMed ID | 8964514 |