Protective effect of ischaemic post-conditioning on ipsilateral and contralateral testes after unilateral testicular ischaemia-reperfusion injury.
Τίτλος | Protective effect of ischaemic post-conditioning on ipsilateral and contralateral testes after unilateral testicular ischaemia-reperfusion injury. |
Publication Type | Journal Article |
Year of Publication | 2011 |
Authors | Shimizu, S., Saito M., Dimitriadis F., Kinoshita Y., Shomori K., Satoh I., & Satoh K. |
Journal | Int J Androl |
Volume | 34 |
Issue | 3 |
Pagination | 268-75 |
Date Published | 2011 Jun |
ISSN | 1365-2605 |
Λέξεις κλειδιά | Animals, Infertility, Male, Ischemic Postconditioning, Male, Malondialdehyde, Oxidative Stress, Peroxidase, Rats, Rats, Sprague-Dawley, Reactive Oxygen Species, Reperfusion Injury, Spermatic Cord Torsion, Testis |
Abstract | The aim of this study was to investigate the effect of ischaemic post-conditioning (IPostC) against ischaemia-reperfusion (IR) injury on bilateral testes after unilateral testicular ischaemia in the rat. Eight-week-old male Sprague-Dawley rats were divided into control group; IR group (60 min ischaemia-24 h reperfusion); IPostC1 × 10 group (60 min ischaemia followed by one cycle of 10 sec reperfusion-10 sec ischaemia; then 24 h reperfusion); IPostC3 × 10 group (three cycles of 10 sec reperfusion-10 sec ischaemia; then 24 h reperfusion); IPostC5 × 10 group (five cycles of 10 sec reperfusion-10 sec ischaemia; then 24 h reperfusion) and IPostC3 × 30 group (three cycles of 30 sec reperfusion-30 sec ischaemia; then 24 h reperfusion). In the IR and IPostC groups, the right testicular vessels were clamped using a special vascular clip. Malondialdehyde (MDA) and myeloperoxidase (MPO) levels were measured in testicular tissue samples bilaterally. Additionally, bilateral testicular tissue samples were processed for histological evaluation including haematoxylin-eosin, 4-hydroxy-2-nonenal (4-HNE) and TdT-mediated dUTP Nick End Labelling (TUNEL) staining. The levels of MDA and MPO as well as the positive cells per seminiferous tubule in TUNEL and 4-HNE stain in bilateral testes from the IR group were significantly higher compared with the control group. IPostC3 × 30 protocol significantly ameliorated the aforesaid parameters in both testes compared with the IR group. For the first time, we have demonstrated that IPostC protects both testes after unilateral testicular ischaemia-reperfusion. IPostC3 × 30 protocol offered the most effective protection. |
DOI | 10.1111/j.1365-2605.2010.01077.x |
Alternate Journal | Int J Androl |
PubMed ID | 20522123 |