Cyclization of PLP peptide reduces its encephalitogenic potential in experimental autoimmune encephalomyelitis.
Title | Cyclization of PLP peptide reduces its encephalitogenic potential in experimental autoimmune encephalomyelitis. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | Lourbopoulos, A., Matsoukas M-T., Katsara M., Deraos G., Giannakopoulou A., Lagoudaki R., Grigoriadis N., Matsoukas J., & Apostolopoulos V. |
Journal | Bioorg Med Chem |
Volume | 26 |
Issue | 9 |
Pagination | 2221-2228 |
Date Published | 2018 May 15 |
ISSN | 1464-3391 |
Abstract | We report the novel synthesis of cyclic PLP (cPLP) and its application in SJL/J mice to study its encephalitogenic effects. Our results indicate that the cPLP analog is minimally encephalitogenic when administered to induce experimental autoimmune encephalomyelitis (low disease burden, minimal inflammatory, demyelinating and axonopathic pathology compared to its linear counterpart). Proliferation assays confirmed the low stimulatory potential of the cPLP compared to linPLP (2.5-fold lower proliferation) as well as inducing lower antibody responses. Molecular modeling showed a completely different TCR recognition profile of cPLP in regard to linPLP, where H replaces W and F-K replace H as TCR contacts, which may explain the difference on each peptide's response. |
DOI | 10.1016/j.bmc.2017.12.024 |
Alternate Journal | Bioorg. Med. Chem. |
PubMed ID | 29681483 |