A novel alpha-galactosidase from Bifidobacterium bifidum with transgalactosylating properties: gene molecular cloning and heterologous expression.
| Title | A novel alpha-galactosidase from Bifidobacterium bifidum with transgalactosylating properties: gene molecular cloning and heterologous expression. |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Goulas, T., Goulas A., Tzortzis G., & Gibson G. R. |
| Journal | Appl Microbiol Biotechnol |
| Volume | 82 |
| Issue | 3 |
| Pagination | 471-7 |
| Date Published | 2009 Mar |
| ISSN | 1432-0614 |
| Keywords | alpha-Galactosidase, Amino Acid Sequence, Bacterial Proteins, Bifidobacterium, Cloning, Molecular, Escherichia coli, Gene Expression, Molecular Sequence Data, Molecular Weight, Open Reading Frames, Sequence Alignment |
| Abstract | A genomic library of Bifidobacterium bifidum (NCIMB 41171) DNA was constructed in Escherichia coli RA11r (melA(-)B(+)) and one alpha-galactosidase encoding gene was isolated. Conceptual translation combined with insertional mutagenesis analysis indicated an open reading frame (ORF) of 759 amino acid (aa) residues encoding an alpha-galactosidase (named as MelA) of 82.8 kDa. Partial purification and characterisation showed that the enzyme had an apparent native molecular mass of approximately 243 kDa and a subunit size of approximately 85 kDa. The enzyme belongs to glycosyl hydrolases 36 family with high aa sequence similarities (approximately 73%) to other known alpha-galactosidases of bifidobacterial origin. Under optimum pH conditions for activity (pH 6.0) and high melibiose concentration (40% w/v), the enzyme was able to form oligosaccharides with degree of polymerisation (DP) > or = 3 at higher concentration than DP = 2, with a total yield of 20.5% (w/w). |
| DOI | 10.1007/s00253-008-1750-5 |
| Alternate Journal | Appl. Microbiol. Biotechnol. |
| PubMed ID | 19005653 |
