Monocyte attachment to laminin in diabetes mellitus: The role of ATP.
Title | Monocyte attachment to laminin in diabetes mellitus: The role of ATP. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Kostidou, E., Trachana V., Topouridou K., Paletas K., Tsapas A., Kaloyianni M., & Koliakos G. |
Journal | Cell Adh Migr |
Volume | 3 |
Issue | 2 |
Pagination | 155-9 |
Date Published | 2009 Apr-Jun |
ISSN | 1933-6926 |
Keywords | Adenosine Triphosphate, Adult, Aged, Case-Control Studies, Cell Adhesion, Diabetes Mellitus, Humans, Laminin, Middle Aged, Monocytes |
Abstract | Monocyte-extracellular matrix interactions have been implicated in atherosclerosis pathophysiology. Laminin, the main basement membrane protein contains cell binding domains that can be cryptic, presented only after protein modification. In the present study we evaluated monocyte attachment to laminin-1 in the presence of ATP. Monocytes were derived from either healthy volunteers or patients with diabetes mellitus type II. For the estimation of monocyte attachment to laminin the myeloperoxidase assay was used. Monocytes derived from diabetic patients, showed an increased ability to attach to laminin (p = 0.0055). The presence of ATP increased the attachment of control monocytes to laminin (p = 0.0022). On the contrary, the presence of ATP did not affect the attachment of monocytes derived from diabetic patients to laminin. Our results indicate a modified interaction between monocytes and laminin-1 in diabetes mellitus. |
Alternate Journal | Cell Adh Migr |
PubMed ID | 19287210 |
PubMed Central ID | PMC2679874 |