Decreased CD3+CD16+ natural killer-like T-cell percentage and zeta-chain expression accompany chronic inflammation in haemodialysis patients.
Title | Decreased CD3+CD16+ natural killer-like T-cell percentage and zeta-chain expression accompany chronic inflammation in haemodialysis patients. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Eleftheriadis, T., Kartsios C., Yiannaki E., Antoniadi G., Kazila P., Pliakos K., Liakopoulos V., & Markala D. |
Journal | Nephrology (Carlton) |
Volume | 14 |
Issue | 5 |
Pagination | 471-5 |
Date Published | 2009 Aug |
ISSN | 1440-1797 |
Keywords | Adult, Aged, Aging, Antigens, CD3, C-Reactive Protein, Chronic Disease, Female, Humans, Inflammation, Interleukin-6, Kidney Failure, Chronic, Killer Cells, Natural, Male, Middle Aged, Receptors, Antigen, T-Cell, Receptors, IgG, Renal Dialysis |
Abstract | AIM: Clinical and experimental data indicate a deficient immune response in haemodialysis (HD) patients. Natural killer-like (NKL) T cells express on their surface both the T-cell antigen receptor (TCR) and a diverse set of NK-cell receptors (NKR) and share properties of both T cells and NK cells. zeta-Chain phosphorylation is an early event that follows TCR activation or some NKR activation. The zeta-chain of both T cell and NK cells is downregulated in many chronic inflammatory states, HD included. In the present study, NKL T-cell percentage and zeta-chain expression in HD patients were evaluated.METHODS: Thirty-three stable HD patients and 30 healthy volunteers were enrolled into the study. NKL T-cell percentage and NKL T-cell zeta-chain mean fluorescence intensity (MFI) were evaluated with flow cytometry. The inflammatory markers C-reactive protein, interleukin-6 and tumour necrosis factor-alpha were measured in the serum by means of enzyme-linked immunosorbent assay.RESULTS: All the evaluated markers of inflammation were increased in HD patients. In these patients, NKL T-cell percentage (1.71 +/- 1.69% vs 3.94 +/- 3.86%) and zeta-chain MFI (3.66 +/- 2.79 vs 7.03 +/- 7.91) were decreased.CONCLUSIONS: NKL T-cell percentage and zeta-chain expression is decreased in HD patients. Taking into consideration the continuously increasing age of the HD patients and that normally NKL T-cell numbers increase with age counteracting the impaired T-cell and NK-cell function accompanying advancing age, the above NKL T-cell disturbances could contribute to the impaired immune response in this population. Measures towards alleviating chronic inflammation could partially restore NKL T-cell impairment. |
DOI | 10.1111/j.1440-1797.2008.01041.x |
Alternate Journal | Nephrology (Carlton) |
PubMed ID | 19486472 |