Evaluation of the "AMR Direct Flow Chip Kit" DNA microarray for detecting antimicrobial resistance genes directly from rectal and nasopharyngeal clinical samples upon ICU admission.
Title | Evaluation of the "AMR Direct Flow Chip Kit" DNA microarray for detecting antimicrobial resistance genes directly from rectal and nasopharyngeal clinical samples upon ICU admission. |
Publication Type | Journal Article |
Year of Publication | 2020 |
Authors | Protonotariou, E., Meletis G., Papadopoulou D., Kachrimanidou M., Toptsi L., & Skoura L. |
Journal | Enferm Infecc Microbiol Clin |
Date Published | 2020 Jul 11 |
ISSN | 1578-1852 |
Abstract | INTRODUCTION: Prompt detection of antibiotic resistance genes in healthcare institutions is of utmost importance in tackling the spread of multi-drug resistant micro-organisms. We evaluated the Antimicrobial Resistance (AMR) Direct Flow Chip Kit versus phenotypic screening assays for rectal and nasopharyngeal specimens upon ICU admission.METHODS: A total of 184 dual specimens (92 rectal and 92 nasopharyngeal swabs) from 92 patients were collected from 11/2017 to 8/2018. All swabs were subjected to both AMR and phenotypic tests according to their origin. The degree of agreement of the two methods was assessed by the kappa coefficient.RESULTS: The kappa coefficient showed perfect agreement for MRSA, ESBLs, oxacillinases and vancomycin resistance genes (1.000, p<0.01) and very good agreement for mecA-positive CoNS, KPC-carbapenemases and metallo-beta-lactamases (0.870, p<0.01; 0.864, p<0.01; and 0.912, p<0.01, respectively).CONCLUSION: The AMR Direct Flow Chip Kit is a useful alternative to phenotypic testing for rapid detection of resistance markers. |
DOI | 10.1016/j.eimc.2020.05.015 |
Alternate Journal | Enferm Infecc Microbiol Clin |
PubMed ID | 32665077 |