Efficient identification of Malassezia yeasts by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).
Title | Efficient identification of Malassezia yeasts by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | Kolecka, A., Khayhan K., Arabatzis M., Velegraki A., Kostrzewa M., Andersson A., Scheynius A., Cafarchia C., Iatta R., Montagna M. T., Youngchim S., Cabañes F. J., Hoopman P., Kraak B., Groenewald M., & Boekhout T. |
Journal | Br J Dermatol |
Volume | 170 |
Issue | 2 |
Pagination | 332-41 |
Date Published | 2014 Feb |
ISSN | 1365-2133 |
Keywords | Dermatomycoses, Gene Library, Humans, Malassezia, Molecular Sequence Data, Reference Values, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
Abstract | BACKGROUND: Infections caused by Malassezia yeasts are most likely underdiagnosed, because fatty acid supplementation is needed for growth. Rapid identification of Malassezia species is essential for appropriate treatment of Malassezia-related skin infections, fungaemia and nosocomial outbreaks in neonates, children and adults and can be life-saving for those patients. Ma-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been reported to be a rapid and reliable diagnostic tool to identify clinically important yeasts, but so far no data have been reported on identification of Malassezia isolates with this technique.OBJECTIVES: To create an extensive database of main mass spectra (MSPs) that will allow quick identification of Malassezia species by MALDI-TOF MS.METHODS: An in-house library of 113 MSPs was created from 48 reference strains from the CBS-KNAW yeast collection. The in-house library was challenged with two test sets of Malassezia strains, namely 165 reference strains from the CBS collection and 338 isolates collected in Greece, Italy, Sweden and Thailand.RESULTS: MALDI-TOF MS allowed correct identification of all 14 Malassezia spp. MALDI-TOF MS results were concordant with those of sequence analyses of the internal transcribed spacers (ITS1/ITS2) and the D1/D2 domains of the large subunit of the ribosomal DNA.CONCLUSIONS: Implementation of the MALDI-TOF MS system as a routine identification tool will contribute to correct identification of Malassezia yeasts with minimal effort and in a short turnaround time, which is especially important for the rapid identification of Malassezia in skin diseases and nosocomial outbreaks. |
DOI | 10.1111/bjd.12680 |
Alternate Journal | Br. J. Dermatol. |
PubMed ID | 24125026 |