We aimed to investigate the association between Red Blood Cell Distribution Width (RDW) and Neutrophil-to-Lymphocyte Ratio (NLR), simple, rapidly assessed markers from the complete blood count with vascular calcification (VC)/stiffness and cardiovascular disease (CVD) in chronic kidney disease (CKD). Dephosphorylated, uncarboxylated matrix Gla-protein (dp-ucMGP), and central/peripheral hemodynamics' parameters were measured in 158 CKD patients, including Hemodialysis and Peritoneal Dialysis. Spearman's rho analysis showed that RDW correlated with C-reactive protein (CRP) (r = 0.29, < 0.001), dp-ucMGP (r = 0.43, = < 0.0001), central diastolic blood pressure (DBP) (r = -0.19, = 0.02), and albuminuria (r = -0.17, = 0.03). NLR correlated with the duration of CVD (r = 0.32, < 0.001), CRP (r = 0.27, = 0.01), dp-ucMGP (r = 0.43, < 0.0001), central DBP (r = -0.32, < 0.0001) and eGFR (r = -0.25, = 0.04). In multiple regression models, circulating dp-ucMGP was an independent predictor of RDW (β = 0.001, = 0.001) and NLR (β = 0.002, = 0.002). In CKD patients, RDW and NLR are associated with traditional and novel markers of VC and CVD.